Objectives

The primary objective of this tender is to procure specialized molecular biology laboratory services for the genetic analysis of twenty biological specimens. The project aims to support taxonomic identification and phylogenetic research by generating reliable DNA sequence data for designated genetic markers.

Scope of Work

The successful supplier will deliver an integrated service covering the entire analytical workflow for twenty specimens. This includes the physical collection of specimens, laboratory-based DNA extraction, polymerase chain reaction (PCR) amplification, and DNA sequencing. The client will provide all necessary primer specifications. The scope encompasses both field logistics and advanced laboratory processing, culminating in the delivery of validated sequence outputs.

Technical Requirements

• Perform high-yield, high-purity DNA extraction from twenty distinct biological specimens.
• Amplify target genomic regions using PCR with provided primer combinations (forward and reverse).
• Conduct DNA sequencing for three specific markers: COI, 16S, and 28S, utilizing five primer sets in total.
• Maintain strict contamination control, chain-of-custody, and sample tracking protocols throughout collection and processing.
• Deliver raw sequencing data, chromatograms, and/or consensus sequences meeting standard quality thresholds.
• Comply with South African public procurement regulations, including mandatory registration on the Central Suppliers Database (CSD) and submission of valid Tax Pin and BBBEE certificates.

Skills Requirements

• Proven expertise in molecular biology techniques, specifically nucleic acid extraction, PCR optimization, and automated/Sanger sequencing.
• Practical experience working with standard molecular taxonomy markers and interpreting multi-region sequence alignments.
• Competence in ethical specimen collection, appropriate preservation methods, and secure transport logistics.
• Ability to troubleshoot low-yield extractions or failed amplifications and implement corrective measures.
• Familiarity with South African preferential procurement frameworks and compliance documentation requirements.

Key Terms & Compliance Notes

COI, 16S, and 28S: These are established genetic markers used in molecular taxonomy. COI (Cytochrome c oxidase subunit I) serves as the standard DNA barcode for animal species identification. 16S and 28S are ribosomal RNA genes typically employed for broader evolutionary relationships and genus-level classification.

Five Primer Sets: Indicates that three target regions will be amplified using multiple forward and reverse primer combinations to ensure complete, bidirectional coverage of each marker.

80/20 Preference Point System: A South African public procurement policy where 80% of evaluation points are awarded for price and functional capability, while 20% are allocated for socio-economic transformation goals, including black ownership, women/youth/disability empowerment, and local municipality participation.

CSD Registration: The Central Suppliers Database is the mandatory national registry for all entities supplying goods or services to South African government departments and public entities. Failure to maintain accurate, up-to-date registration may result in disqualification.