Analysis – Proteomics Analysis by Lc-ms.

INF012510RFQ14

See details below or the tender documentation

Tender Closed on: 2025-10-20 11:00

Laboratory, Testing and Analytical Services

Stellenbosch, Western Cape

Agricultural Research Council

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Agricultural Research Council Tenders

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Tender Number: INF012510RFQ14
Department: Agricultural Research Council
Tender Type: Request for Quotation
Province: Western Cape
Closing Date: Monday, 20 October 2025 - 11:00
Place where goods, works or services are required: AGRICULTURAL RESEARCH COUNCIL NIETVOORBIJ, R 44 KLAPMUTS ROAD - CLOETESVILLE - Stellenbosch - 7160
Special Conditions: N/A
ENQUIRIES:
Contact Person: ZIMKHITA NATACIA MATAYI
Email: [email protected]
Telephone number: 021-809-3093
FAX Number: N/A
BRIEFING SESSION:
Is there a briefing session?: NO Is it compulsory? NO
Briefing Date and Time:
Briefing Venue:

This tender is also available from www.etenders.gov.za


Tender Summary

Objectives

The primary objective of this tender is to conduct proteomic profiling of grape berry skins using LC-MS/MS technology. The goal is to evaluate the impact of sheets coated with essential oils—specifically lemon and peppermint oils—as a replacement for sulphur dioxide. This biocontrol mechanism aims to inhibit Botrytis cinerea during cold storage and shipping of table grapes.

Scope

The scope includes:

  • Analysis of 90 pallet form samples for quantification, digestion, and LC-MS analysis.
  • Sampling at three time points: 5 weeks, 7 weeks, and 9 weeks.
  • Six treatments per time point, with five replicates per condition, totaling 15 samples per treatment.
  • Analysis of sheets coated with lemon oil and peppermint oil at specified intervals.

Technical Requirements

The technical specifications mandate:

  • Compliance with the provided specifications, with deviations clearly indicated if any.
  • Preparation and analysis of samples using LC-MS/MS technology.
  • Processing of samples at three designated time points with multiple treatments and replicates.
  • Adherence to quality standards for quantification and digestion procedures.

Skills Requirements

The successful bidder must demonstrate:

  • Proven experience in proteomic analysis using LC-MS/MS.
  • Capability to handle sample preparation, digestion, and quantification for complex biological samples.
  • Experience in working with plant tissue samples, specifically grape berry skins, is preferred.
  • Ability to interpret proteomic data and generate comprehensive reports.
  • Compliance with administrative and quality standards as specified in the tender documentation.
This summary is AI generated. Download the tender documents for all the information.

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